Bio-Prodict 3DM

Bio-Prodict is focused on delivering solutions for guiding scientific research in the field of protein engineering, molecular design and DNA diagnostics. We apply novel approaches to data mining, storage and analysis of protein data and combine these with state-of-the art analysis methods and visualization tools to create custom-built information systems for protein superfamilies.

Over the years, these systems have proved to be powerful instruments for understanding many of the complex aspects involved in protein function and mutation effect prediction.

Years of experience in software design, structural biology and the analysis of protein super-family data make Bio-Prodict your choice for protein-related data analysis and software design.

We have released a special version of 3DM that is tailored toward DNA Diagnostics. 3DM for DNA Diagnostics provides predictions about the pathogenicity of variants and helps you deal with the growing amounts and complexity of biomedical data. Curious? Find out more!

The 3DM intro below highlights some of the key concepts and functions of our protein superfamily data integration platform, 3DM.

If you would like to try 3DM you can register for a 3DM demo account.


News

Bio-Prodict was selected to take part in two large European grants: SUZY and BIOOX, which will start October 1st.

Bio-Prodict has been awarded a FD Gazellen award 2012! This prize is awarded to the fastest growing companies in the Netherlands.


Technology

The main products of Bio-Prodict are 3DM information systems. 3DM information systems are protein super-family platforms that collect, combine and integrate many different types of protein-related data. 3DM systems are designed to facilitate the exploration of sequence-structure-function relations, and have successfully been used many times to elucidate the function of individual amino acids, predict the effects of mutations, among others. We have a number of publicly available information systems that you can try out.

Data management

For any one protein family there is already an overwhelming amount of data available. Managing and integrating all this data can be a daunting task; studies that involve carrying over information from one protein to the other seem simple at a first glance, however, the amount of data that needs to be collected from heterogeneous sources, converted to syntactic and semantic homogeneity, validated, curated, stored and indexed, is enormous. To manage the increasing amounts of protein-related data Bio-Prodict has developed 3DM: systems that can automatically collect, store and connect many different super-family data types from various sources. Examples of data types that are used by 3DM are sequences, structural information, protein-ligand and protein-protein contact information, mutational data (which is automatically retrieved from literature), and information extracted from multiple sequence alignments (correlated mutations, amino acid conservation patterns, subfamily specific residues, etc). All these different data types are stored in specialized protein super-family system that are centered around a structure based multiple sequence alignment.

Data integration

3DM applies a unified numbering scheme to the alignment, the sequences, and the structures. This numbering scheme makes it easy to visualize and integrate all different data types in the alignment, in the structures and in relation to each other, enabling easy detection of correlations between the different data types. Using a common numbering scheme for all proteins in the system allows for the easy transfer of information between proteins. 3DM super-family systems have a highly flexible setup making it easy to implement in-house data (such as mutational information).

3DM is tightly integrated with the state of the art molecular graphics and modeling software YASARA , which makes it easy to project the different data types on a protein structures and visualize trends extracted from the 3DM systems in a structural context. The use of YASARA, winner of the CASP8 refinement section, to build 3DM-based homology models ensures that you get the highest quality models possible.

Relating information between databases and literature has become increasingly difficult with the growing quantities of data and documents available. With the goal of bridging the gap between data and scientific articles, we have fully integrated the Utopia Documents PDF reader and the 3DM information systems. The software presents you with current, integrated, validated, internally consistent data and information in the context of literature being read. The annotations provided are extensive and detailed, and offer one-click access to related literature and sequential and structural information. 3DM offers full-circle data integration; you can navigate seamlessly between sequential data, protein structures and literature.


3DM course

The 3DM course will give an overview of what 3DM information systems can and cannot do when applied to problems in Protein Engineering, Homology Modeling and Drug Design. The participants will explore different kinds of protein super-family data and will learn how to use and extract relations from the data. The structure visualization tool YASARA will be used to deal with the data from a structural perspective, whereas Utopia Documents will show you how the data in the 3DM systems is integrated with full-text literature. The course contains a large practical component in which the participants learn to work with the tools using real-life protein engineering and drug design examples.

We expect the next course will be scheduled for February or March of 2014, depending on demand.
Costs: (PhD) Student: €300; Academic: €400; Commercial: €600

Register
Course schedule
Course materials
Location: CMBI, Nijmegen, the Netherlands


Contact

Address

Bio-Prodict BV
Nieuwe Marktstraat 54E
6511 AA, Nijmegen
The Netherlands
P: +31 (0)24 845 7988
M: +31 (0)6 5354 8336
E: Info at Bio-Prodict dot NL

3DM Demo

3DM comes in two flavours: 3DM, and 3DM for DNA Diagnostics.


Publications

2014:

1. GPCRDB: an information system for G protein-coupled receptors. (NAR)

2013:

3DM was used in a series of protein engineering experiments:

1. Use of 'small but smart' libraries to enhance the enantioselectivity of an esterase from Bacillus stearothermophilus towards tetrahydrofuran-3-yl acetate. (FEBS J)

2. Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning. (Elsevier)

3. Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input. (Elsevier)

Protein engineering books and reviews refering to 3DM:

4. Site-Directed Mutagenesis as Applied to Biocatalysts. (springer)

5. Protein Engineering as a Tool for the Development of Novel Bioproduction Systems. (CDN)

6. Engineering proteins for thermostability through rigidifying flexible sites (RFS). (Biotechnol Adv)

2012:

Drug design is becoming more and more difficult and 3DM has been described as an essential tool for drug design in the future.

1. Drug design for ever, from hype to hope (J Comput Aided Mol Des)

2. Recent advances in rational approaches for enzyme engineering (CSBJ)

Protein engineering books and reviews refering to 3DM:

3. Strategies for discovery and improvement of enzyme function: state of the art and opportunities. (Microb Biotechnol)

4. Hydrolases as Catalysts for Green Chemistry and Industrial Applications-Esterase, Lipase and Phytase. (PhD thesis)

2011:

3DM was used to increase the thermostability by rational design of a sucrose phosphorylase.

1. Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis (PEDS)

Utopia (a pdf reader that can analyse the content of publications) is coupled to 3DM enabling visualisation of 3DM data in articles and provides links to relavant literature.

2. Integrating GPCR-specific information with full text articles. (BMC bioinformatics)

3. NucleaRDB:information system for nuclear receptors. (NAR)

A 3DM system was generated for the P450 protein super-family and it's applicability for P450 related research is extensively described.

4. 3DM Protein Engineering Super-Family systems applied to the P450 family. (Proceedings of the 17th International Conference on Cytochrome P450)

3DM was used to guide the discovery of a compensating mutation in C-methyltransferase NovO.

5. Molecular characterization of the C-methyltransferase NovO of Streptomyces spheroides, a valuable enzyme for performing Friedel-Crafts alkylation. (J. Mol. Catal. B: Enzym)

2010:

3DM was used to investigate the alpha-beta-hydrolase protein super-family, which revealed specific motifs that divides the different functional members. Furthermore, this 3DM was used to design specific libraries that have a high content of mutants with increased activity (250x), enantioselectivity (E3 -> E80), and thermostability. The results have been published in four articles:

1. The a-b-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering (Chembiochem)

2. Thermostabilization of an esterase by alignment-guided focussed directed evolution ( PEDS)

3. Natural Diversity to Guide Focused Directed Evolution (Chembiochem)

4. Simulation on the structure of pig liver esterase. (J Mol Model)

A review of the 3DM methodology has been published in Proteins. This review shows how 3DM can guide experimental design, demonstrated with results obtained from different 3DM systems.

5. 3DM: systematic analysis of heterogeneous super-family data to discover protein functionalities. (Proteins)

To predict pathogenicity of mutations in disease-related proteins, two new 3DM tools have been developed: Validator (a graphical user interface for DNA diagnostics) and Mutator (a tool for automatic collection of mutational data from literature). These have been used to generate the FMDB (Fabry Mutation Database)

6. Novel tools for extraction and validation of disease related mutations applied to Fabry disease. (Human Mutation) (1 editorial note)

A novel DNA diagnostics mutation prediction tool was developed (HOPE) and tested on 16 human disease-related proteins.

7. Protein structure analysis of mutations causing inheritable diseases. An e-Science approach with life scientist friendly interfaces. (BMC Bioinformatics)

In 2010 three reviews were published describing current protein engineering strategies, describing 3DM as a powerful tool.

8. Beyond directed evolution--semi-rational protein engineering and design. (Curr Opin Biotechnol)

9. Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst. (Curr Opin Chem Biol)

10. Protein design in metabolic engineering and synthetic biology. (Curr Opin Biotechnol)

2009:

3DM contains multiple novel super-family analysis tools, such as a correlated mutation analysis tool (Comulator) specifically designed for the analysis of large super-family alignments. Comulator was used to increase enzyme activity and change enzyme specificity, and proved a powerful tool in enzyme design:

1. Correlated mutation analysis on super-family alignments reveal functionally important residues. (Proteins)

2. Identification of a gatekeeper residue that prevents dehydrogenases to act as oxidases. (JBC)

2008:

The first automatically generated 3DM system was created for the isocitrate lyase superfamily. Oxaloacetate hydrolase (OAH) is a member of this enzyme super-family. This 3DM was used to investigate OAH-like proteins and to design mutagenesis experiments. This work revealed the unknown reaction mechanism of OAH, which enabled the design of a strong inhibitor. Furthermore, it was shown that 3DM could be used to find hotspots for enzyme specificity. This in turn predicted a new function for 2,3-Dimethylmalate Lyase, a protein highly similar to OAH. The results are published in three peer-reviewed articles:

1. Oxaloacetate Hydrolase, the C-C Bond Lyase of Oxalate Secreting Fungi. (JBC)

2. Identification of fungal oxaloacetate hydrolyase within the isocitrate lyase/PEP mutase enzyme superfamily using a sequence marker-based method. (Proteins)

3. Structure and Function of 2,3-Dimethylmalate Lyase, a PEP Mutase/Isocitrate Lyase Superfamily Member. (JMB)

2007:

3DM was developed because the generation and updating of super-family systems is very time consuming. The 3DM method is patented in 2007 and fully described in the PhD thesis of Henk-Jan Joosten:

1. Method of generating a protein database. (patent WO/2008/035970)

2. 3DM: From Data to Medicine. (PhD thesis)

2004-2005:

Superfamily information systems have been guiding the understanding of different aspects of protein function. Folketsma et al. showed how to use such systems to understand the function of specific amino acids:

1. A family-based approach reveals the function of residues in the nuclear receptor ligand-binding domain (JMB)

2. The Nuclear Receptor Ligand-Binding Domain: A Family-Based Structure Analyses. (Current Med. Chem.)


About us

Bio-Prodict is a private Dutch computational biotechnology company founded 5th of May 2008.

The core business of Bio-Prodict is development of protein superfamily systems aimed at speeding up research time in the area of drug design, protein engineering, and DNA diagnostics.

Bio-Prodict uses the ICT~Office Terms and Conditions.


Team


Dr. Henk-Jan Joosten
joosten@bio-prodict.nl,
+31 (0)6-5354 8336

Managing director (CEO)

Dr. Remko K.P. Kuipers
rkuipers@bio-prodict.nl
Senior software developer

Tom van den Bergh, MSc.
tvandenbergh@bio-prodict.nl
Project manager
3DM development

Dr. Bas Vroling
bvroling@bio-prodict.nl
Diagnostics project manager

Tim A.H. te Beek, Bachelor of ICT
ttebeekbio-prodict.nl
Senior software developer

Positions & Internships

We're currently looking for a skilled software developer to expand our team for a broad mix of front- and backend development. If you would like to know more please contact Henk-Jan Joosten.

We continuously offer internships to bright young students in bio-informatics and computer science.

For more information send us an email.


Board

Dr. H.J. Joosten
Managing director
Dr. H.J. Joosten is one of the founders of Bio-Prodict. He got a masters degree in bio-informatics in 2002 at the Centre of Molecular and Bio-molecular Informatics (CMBI) where he worked on the generation of a protein super-family database for the nuclear receptor super-family.
He completed his PhD in 2006 at the Wageningen University where he developed 3DM. He continued the development of 3DM during a post-doctoral period till December 2007. From the start of 2008 he focused on the foundation of Bio-Prodict resulting in the startup in May 2008.

Ir. P.A.M. Heemskerk
Chairman and advisor
Ir. P.A.M. Heemskerk graduated in 1971 in Food Science and Technology at Wageningen University. He spent his working life in the food and beverage industry notably with the Heineken beer company. Ir. Heemskerk has extensive experience in production, supply chain, (bio)technology as well as management development. His main competencies: strategy, innovation, integrating perspectives and cultures, teambuilding and coaching. His international business skills were built up across all continents, and he is driven to coach the director of Bio-Prodict to develop a successful company.

Prof. Dr. G. Vriend
Scientific advisor
Professor Vriend got his PhD in 1983 at the agricultural university of Wageningen. After doing post-doctoral research in Purdue, India, USA and in Groningen, The Netherlands, he worked at the EMBL in Heidelberg, Germany. In 1999, he founded the Centre of Molecular and Bio-molecular Informatics (CMBI) in Nijmegen, The Netherlands. At the CMBI, he continued working on fundamental and applied aspects of protein structure (homology modelling, structure quality, visualization, molecular dynamics, ligand interactions) and on information technology (data-mining and information systems).

Wageningen Business Generator
Co-founder
The Wageningen Business Generator (WBG) is an organization responsible for valorization of science within the Wageningen University. WBG supports new spin-offs with their legal, scientific, financial and patent expertise and give financial support. WBG has largely funded the start of Bio-Prodict.


Our partners & customers

Bio-Prodict is partner in serveral EU projects


Kyrobio: developing biocatalysts for industrial chiral chemicals


Newprot: Interactive protein engineering portal with validated software and database facilities.


MicroB3: Biodiversity, Bioinformatics, Biotechnology... Innovation from the sea.


Neuromics: Integrated European Project on Omics Research of Rare Neuromuscular and Neurodegenerative Diseases


BIOOX: Developing a validated technology platform for the application of oxygen dependent enzymes in synthesis and transformation of alcohols


SuSy: Sucrose Synthase as Effective Mediator of Glycosylation